For a given enzyme catalyzed reaction, the Michaelis constant is 0.6mM and the substrate concentration is 1.0mM. What is the fractional saturation of the enzyme under these conditions?

For a given enzyme catalyzed reaction, the Michaelis constant is 0.6mM and the substrate concentration is 1.0mM. What is the fractional saturation of the enzyme under these conditions?

Options 

A) 60.0%

B) 62.5%

C) 50%

D) 37.5%

E) 40.0%

The fractional saturation of an enzyme is defined as the amount of enzyme that is bound to substrate divided by the total amount of enzyme. To calculate the fractional saturation, we'll need to use the Michaelis-Menton equation:

In addition, we'll need to define the rate and maximum rate in terms of enzyme concentrations:

Vo=kcat[ES]    &  Vmax=kcat[E]T

From the above equations, we can calculate the fractional saturation of the enzyme:

so the correct option is 62.5%