Estimation of Polyphenols
Polyphenols are a group of natural compounds found in plants that have antioxidant properties and may offer several health benefits. Estimation of polyphenols in a given sample can be done through various methods, including:
Folin-Ciocalteu method: This method is based on the reaction of polyphenols with the Folin-Ciocalteu reagent, which produces a blue color that can be measured spectrophotometrically. This method is widely used for total polyphenol estimation.
HPLC method: High-performance liquid chromatography (HPLC) is a highly sensitive and specific method for the separation and quantification of polyphenols. It is often used to identify and quantify individual polyphenolic compounds in a sample.
UV-Vis spectrophotometry: This method is based on the absorption of UV and visible light by polyphenolic compounds. It can be used for the quantitative estimation of total polyphenols in a sample.
Fluorescence spectrometry: This method is based on the fluorescence emitted by polyphenolic compounds when they are excited by a specific wavelength of light. It is often used for the identification and quantification of specific polyphenolic compounds.
The choice of method depends on the type and complexity of the sample, the availability of equipment, and the purpose of the analysis.
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